期刊
PLANT PHYSIOLOGY AND BIOCHEMISTRY
卷 57, 期 -, 页码 54-58出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.plaphy.2012.04.014
关键词
Hypericum perforatum; Xanthones; Three-step root culture system; Candida species; Cryptococcus neoformans; Dermatophytes
资金
- Italian Ministry of Instruction, Universities and Research [Reti FIRB RBPR05NWWC_006]
Hypericum perforatum is a well-known medicinal plant. Among all secondary metabolites produced by this species, xanthones are very interesting for their antifungal activity. In the present study, with the aim to improve xanthone production and antifungal activity of H. perforatum subsp. angustifolium (sin. Frohlich) Borkh in vitro roots, a new methodology consisting of a three-step culture system, has been developed. Regenerated roots of H. perforatum were cultured in a three-step culture system: in the first step, to increase biomass, the roots were cultured in half-strength liquid Murashige and Skoog (MS) medium supplemented with 1 mg L-1 indole butyric acid (IBA) and 1.5% sucrose. In the second and third steps, to stimulate secondary metabolism, the roots were cultured with 1.1 mg L-1 2,4-dichlorophenoxyacetic acid (2,4-D), 0.215 mg L-1 kinetin (KIN), and 0.186 mg L-1 1-naphthalenacetic acid (NAA). In the third step, some of the roots were treated with chitosan. Xanthone production increased 2.7 times following the three-step method. The mean minimal inhibitory concentration (MIC) values were of 36.9, 26.7, and 65 mu g mL(-1), against Candida species, Cryptococcus neoformans and dermatophytes, respectively. A positive correlation between xanthone accumulation and antifungal activity has been shown. (C) 2012 Elsevier Masson SAS. All rights reserved.
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