期刊
PLANT PHYSIOLOGY AND BIOCHEMISTRY
卷 58, 期 -, 页码 124-134出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.plaphy.2012.06.018
关键词
Cadmium; Lupine; Nitric oxide; Programmed cell death; Reactive nitrogen species; Reactive oxygen species; Stress response
资金
- Ministry of Science and Higher Education [N N303303634]
The sequence of events leading to the programmed cell death (PCD) induced by heavy metals in plants is still the object of extensive investigation. In this study we showed that roots of 3-day old yellow lupine (Lupinus luteus L.) seedlings exposed to cadmium (Cd, 89 mu M CdCl2) resulted in PCD starting from 24 h of stress duration, which was evidenced by TUNEL-positive reaction. Cd-induced PCD was preceded by a relatively early burst of nitric oxide (NO) localized mainly in the root tips. Above changes were accompanied by the NADPH-oxidase-dependent superoxide anion (Or) production. However, the concomitant high level of both NO and O-2(center dot-) at the 24th h of Cd exposure did not provoke an enhanced peroxynitrite formation. The treatment with the NADPH-oxidase inhibitor and NO-scavenger significantly reduced O-2(center dot-) and NO production, respectively, as well as diminished the pool of cells undergoing PCD. The obtained data indicate that boosted NO and O-2(center dot-) production is required for Cd-induced PCD in lupine roots. Moreover, we found that in roots of 14-day old lupine plants the NO-dependent Cd-induced PCD was correlated with the enhanced level of the post-stress signals in leaves, including distal NO cross-talk with hydrogen peroxide. (C) 2012 Elsevier Masson SAS. All rights reserved.
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