期刊
PLANT PHYSIOLOGY AND BIOCHEMISTRY
卷 49, 期 10, 页码 1244-1249出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.plaphy.2011.08.003
关键词
Vitamin C; Ascorbate; Tomato; MDHAR; DHAR
资金
- A Plant Sciences Departmental GSR
- Rockefeller Foundation
- Plant Powered Production (P3) Center
- NSF [EPS-0701890]
- Arkansas Biosciences Institute
- NIH [P20-RR-016460]
- National Center for Research Resources
Vitamin C (L-ascorbate, MA) is an essential nutrient required in key metabolic functions in humans and must be obtained from the diet, mainly from fruits and vegetables. Given its importance in human health and plant physiology we sought to examine the role of the ascorbate recycling enzymes monodehydroascorbate reductase (MDHAR) and dehydroascorbate reductase (DHAR) in tomato (Solanum lycopersicum), an economically important fruit crop. Cytosolic-targeted tomato genes Mdhar and Dhar were cloned and over-expressed under a constitutive promoter in tomato var. Micro-Tom. Lines with increased protein levels and enzymatic activity were identified and examined. Mature green and red ripe fruit from DHAR over-expressing lines had a 1.6 fold increase in MA content in plants grown under relatively low light conditions (150 mu mol m(-2) s(-1)). Conversely, MDHAR over-expressers had significantly reduced AsA levels in mature green fruits by 0.7 fold. Neither over-expressing line had altered levels of MA in foliar tissues. These results underscore a complex regulation of the MA pool size in tomato. (C) 2011 Elsevier Masson SAS. All rights reserved.
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