期刊
PLANT PHYSIOLOGY AND BIOCHEMISTRY
卷 49, 期 12, 页码 1384-1391出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.plaphy.2011.09.012
关键词
OsDREB2A; 4ABRC promoter; Stress; Transgenic rice
资金
- National Natural Science Foundation of China [30870214]
- China National Major scientific projects [2009ZX08009-017B, 2008ZX001-003]
- Natural Science Foundation of Hebei Province in China [C2010000385]
- Hebei Provincial Education Department [2007469]
The DREB and CBF transcription factors play a critical role in plant development and abiotic stress responses and, therefore, represent attractive targets for a molecular plant breeding approach. In this study, the rice OsDREB2A gene was isolated and expressed under the control of a stress-inducible promoter (4ABRC) to improve the abiotic stress tolerance of japonica rice variety TNG67. T(2) and T(3) transgenic lines over-expressing OsDREB2A were found to have improved survival rates under severe drought and salt stress conditions relative to non-transgenic rice plants or rice plants transformed with the empty vector control. OsDREB2A expression was found to be markedly induced by drought and ABA treatment. The results indicate that the induced over-expression of OsDREB2A driven by the 4ABRC promoter in engineered rice plants may protect cells during stress. (C) 2011 Elsevier Masson SAS. All rights reserved.
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