期刊
PLANT PHYSIOLOGY AND BIOCHEMISTRY
卷 48, 期 4, 页码 216-224出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.plaphy.2010.01.015
关键词
Ascorbate; Formation; Gene expression; L-galactose pathway; Malus leaves
资金
- Natural Science Fund of China [30971971]
- Talent Support Program of Northwest AF University
Ascorbic acid (AsA), as a unique antioxidant and enzyme cofactor, has multiple roles in plants. However, there is very limited information on the mechanism of AsA accumulation and controlling in leaves. In this study, we determined AsA accumulation levels, analyzed expression patterns of the genes involved in synthesizing via L-galactose pathway and recycling as well as enzyme activities in apple (Malus domestica Borkh) leaves with different age. AsA content was found to increase with leaf development, reaching the highest level in 20-day-old leaves. This level was maintained in mature leaves until the dropping in senescent leaves. Comparing with young and senescent leaves, mature leaves had higher capability for AsA synthesis with high expression levels and activity of L-galactose dehydrogenase and L-galactono-1,4-lactone dehydrogenase. The mRNA expression of genes involved in AsA synthesis also showed highest abundance in 20-day-old leaves, though GDP-mannose-3',5'-epimerase and L-galactose-1-phosphate phosphatase expression reached the highest levels before 20 days old. These results suggest that AsA accumulation in apple leaves mainly occurs during the transition phase from young to mature leaves with high rates of synthesis and recycling, and that L-galactose-1-phosphate phosphatase could play an important role in regulating AsA biosynthesis via the L-galactose pathway. (c) 2010 Elsevier Masson SAS. All rights reserved.
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