期刊
PLANT PHYSIOLOGY AND BIOCHEMISTRY
卷 47, 期 8, 页码 724-731出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.plaphy.2009.03.009
关键词
Oligochitosan induced protein kinase; Transgenic tobacco; TMV resistance; Pathogenesis related proteins
资金
- National Natural Science Foundation of China [10775038, 10645006, 10647121]
- Hi-Tech Research and Development Program of China [2006AA10A 213, 2007AA091601]
- Knowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-YW-N-007]
Oligochitosan (OC) can regulate plant defense responses in many aspects, but the basic signal transduction pathway is still unclear. In this study, we used transgenic (TG) tobacco (Nicotiana Tabacum var. Samsun NN) as plant material whose oligochitosan induced protein kinase (OIPK) gene was inhibited by antisense transformation, to study the role of OIPK in tobacco defense reactions. The results showed that OIPK could increase tobacco resistance against tobacco mosaic virus (TMV), in that wild-type (WT) tobacco showed longer lesion appearance time, higher lesion inhibition ratio, smaller average final lesion diameter and lower average final lesion area percent to whole leaf area. It led us to analyze some pathogenesis related (PR) enzymes' activities and mrRNA level, which played roles in tobacco resistance against TMV. We found that phenylalanine ammonia-lyase (PAL) and peroxidase (POD) activities were positively related to OIPK, but not polyphenol oxidase (PPO). It was also demonstrated that OIPK mRNA could be induced by OC, wound and TMV infection. In addition, OIPK could up-regulated three PR genes, PAL, chitinase (CHI) and beta-1, 3-glucanase (GLU) mRNA level to different extent. Taken together, these results implied that OIPK could function in tobacco resistance against both biotic and abiotic stress, possibly via various PR proteins. (C) 2009 Elsevier Masson SAS. All rights reserved.
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