期刊
PLANT PHYSIOLOGY AND BIOCHEMISTRY
卷 47, 期 1, 页码 26-30出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.plaphy.2008.09.007
关键词
Arabidopsis thaliana; beta-Amyrin synthase; At1g78950; Oxidosqualene cyclase
资金
- Ministry of Education, Science, Culture, and Sports of Japan [18032024]
- Japan Society for the Promotion of Science [15101007]
- Takeda Science Foundation
Triterpene skeletons are produced by oxidosqualene cyclases (OSCs). The genome sequencing of Arabidopsis thaliana revealed the presence of thirteen OSC homologous genes including At1g78950, which has been revised recently as two independent ORF's, namely At1g78950 and At1g78955. The cDNA corresponding to the revised At1g78950 was obtained by RT-PCR, ligated into Saccharomyces cerevisiae expression vector pYES2, and expressed in a lanosterol synthase deficient S. cerevisiae strain. LC-MS and NMR analyses of the accumulated product in the host cells showed that the product of At1g78950 is beta-amyrin, indicating that At1g78950 encodes a beta-amyrin synthase (EC 5.4.99.-). (C) 2008 Elsevier Masson SAS. All rights reserved.
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