期刊
PLANT PHYSIOLOGY AND BIOCHEMISTRY
卷 47, 期 5, 页码 377-383出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.plaphy.2009.01.006
关键词
Barley (Hordeum vulgare L); ZIP transporters; Zinc; Transport; Metal homeostasis; Yeast
资金
- Ministry of Science, Technology and Innovation [274-06-0325]
- European Union Framework Programme
Zinc (Zn) is an essential element for plants but limited information is Currently available on the molecular basis for Zn2+ transport in crop species. To expand the knowledge on Zn2+ transport in barley (Hordeum vulgare L.), a cDNA library prepared from barley roots was expressed in the yeast (Saccharomyces cerevisiae) mutant strain Delta zrt1/Delta zrt2, defective in Zn2+ uptake. This strategy resulted in isolation and identification of three new Zn2+ transporters from barley. All of the predicted proteins have a high similarity to the ZIP protein family, and are designated HvZIP3, HvZIP5 and HvZIP8, respectively. Complementation studies in Delta zrt1/Delta zrt2 showed restored growth of the yeast cells transformed with the different HvZIPs, although with different efficiency. Transformation into Fe2+ and Mn2+ uptake defective yeast mutants showed that the HvZIPs were unable to restore the growth on Fe2+ and Mn2+ limited media, respectively, indicating a specific role in Zn 2;, transport. In intact barley roots, HvZIP8 Was constitutively expressed whereas HvZlP3 and HvZIP5 were mainly expressed in -Zn plants. These results Suggest that HvZIP3, HvZIP5 and HvZIP8 are Zn 2 1 transporters involved in Zn2+ homeostasis in barley roots. The new transporters may facilitate breeding of barley genotypes with improved Zn efficiency and Zn content. (C) 2009 Elsevier Masson SAS. All rights reserved.
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