期刊
PLANT PHYSIOLOGY
卷 167, 期 1, 页码 60-+出版社
AMER SOC PLANT BIOLOGISTS
DOI: 10.1104/pp.114.248450
关键词
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资金
- Ruth and Herman Albert Scholars Program for New Scientists
- Charles and Louise Gartner Fund
- Alternative Energy Research Initiative Center at the Weizmann Institute
The halotolerant green alga Dunaliella bardawil is unique in that it accumulates under stress two types of lipid droplets: cytoplasmatic lipid droplets (CLD) and beta-carotene-rich (beta C) plastoglobuli. Recently, we isolated and analyzed the lipid and pigment compositions of these lipid droplets. Here, we describe their proteome analysis. A contamination filter and an enrichment filter were utilized to define core proteins. A proteome database of Dunaliella salina/D. bardawil was constructed to aid the identification of lipid droplet proteins. A total of 124 and 42 core proteins were identified in beta C-plastoglobuli and CLD, respectively, with only eight common proteins. Dunaliella spp. CLD resemble cytoplasmic droplets from Chlamydomonas reinhardtii and contain major lipid droplet-associated protein and enzymes involved in lipid and sterol metabolism. The beta C-plastoglobuli proteome resembles the C. reinhardtii eyespot and Arabidopsis (Arabidopsis thaliana) plastoglobule proteomes and contains carotene-globule-associated protein, plastid-lipid-associated protein-fibrillins, SOUL heme-binding proteins, phytyl ester synthases, beta-carotene biosynthesis enzymes, and proteins involved in membrane remodeling/lipid droplet biogenesis: VESICLE-INDUCING PLASTID PROTEIN1, synaptotagmin, and the eyespot assembly proteins EYE3 and SOUL3. Based on these and previous results, we propose models for the biogenesis of beta C-plastoglobuli and the biosynthesis of beta -carotene within beta C-plastoglobuli and hypothesize that beta C-plastoglobuli evolved from eyespot lipid droplets.
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