期刊
PLANT PHYSIOLOGY
卷 161, 期 3, 页码 1202-1216出版社
AMER SOC PLANT BIOLOGISTS
DOI: 10.1104/pp.112.205385
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资金
- Japan Society for the Promotion of Science [18-06619]
- Ministry of Agriculture, Forestry, and Fisheries (Genomics for Agricultural Innovation and Development of Abiotic Stress Tolerant Crops by DREB Genes)
- Program for the Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry in Japan
OsTZF1 is a member of the CCCH-type zinc finger gene family in rice (Oryza sativa). Expression of OsTZF1 was induced by drought, high-salt stress, and hydrogen peroxide. OsTZF1 gene expression was also induced by abscisic acid, methyl jasmonate, and salicylic acid. Histochemical activity of beta-glucuronidase in transgenic rice plants containing the promoter of OsTZF1 fused with b-glucuronidase was observed in callus, coleoptile, young leaf, and panicle tissues. Upon stress, OsTZF1-green fluorescent protein localization was observed in the cytoplasm and cytoplasmic foci. Transgenic rice plants overexpressing OsTZF1 driven by a maize (Zea mays) ubiquitin promoter (Ubi: OsTZF1-OX [for overexpression]) exhibited delayed seed germination, growth retardation at the seedling stage, and delayed leaf senescence. RNA interference (RNAi) knocked-down plants (OsTZF1-RNAi) showed early seed germination, enhanced seedling growth, and early leaf senescence compared with controls. Ubi: OsTZF1-OX plants showed improved tolerance to high-salt and drought stresses and vice versa for OsTZF1-RNAi plants. Microarray analysis revealed that genes related to stress, reactive oxygen species homeostasis, and metal homeostasis were regulated in the Ubi: OsTZF1-OX plants. RNA-binding assays indicated that OsTZF1 binds to U-rich regions in the 39 untranslated region of messenger RNAs, suggesting that OsTZF1 might be associated with RNA metabolism of stress-responsive genes. OsTZF1 may serve as a useful biotechnological tool for the improvement of stress tolerance in various plants through the control of RNA metabolism of stress-responsive genes.
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