期刊
PLANT PHYSIOLOGY
卷 150, 期 3, 页码 1459-1473出版社
OXFORD UNIV PRESS INC
DOI: 10.1104/pp.109.140905
关键词
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资金
- Crop Functional Genomics Center of the 21st Century Frontier Research Program [CG2151]
- BioGreen 21 Program [20070401034022]
- Rural Development Administration, Korea Science and Engineering Foundation [R01-2007-000-10041-0]
- Korea Science and Engineering Foundation Environmental Biotechnology Research Center [R15-2003-012-02003-0]
- National Research Foundation of Korea [R15-2003-012-02003-0, R01-2007-000-10041-0, 과06A1204, 2007-0052422] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- Rural Development Administration (RDA), Republic of Korea [20070401034022, 07-2007-07-001-00] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Understanding the cellular differentiation of multicellular organisms requires the characterization of genes whose expression is modulated in a cell type-specific manner. The Arabidopsis (Arabidopsis thaliana) root hair cell is one model for studying cellular differentiation. In this study, root hair cell-specific genes were screened by a series of in silico and experimental filtration procedures. This process included genome-wide screening for genes with a root hair-specific cis-element in their promoters, filtering root-specific genes from the root hair-specific cis-element-containing genes, further filtering of genes that were suppressed in root hair-defective plant lines, and experimental confirmation by promoter assay. These procedures revealed 19 root hair-specific genes, including many protein kinases and cell wall-related genes, most of which have not been characterized thus far. Functional analyses of these root hair-specific genes with loss-of-function mutants and overexpressing transformants revealed that they play roles in hair growth and morphogenesis. This study demonstrates that a defined cis-element can serve as a filter to screen certain cell type-specific genes and implicates many new root hair-specific genes in root hair development.
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