期刊
PLANT PATHOLOGY JOURNAL
卷 30, 期 4, 页码 343-354出版社
KOREAN SOC PLANT PATHOLOGY
DOI: 10.5423/PPJ.OA.06.2014.0055
关键词
defense response; Magnaporthe oryzae; MapMan analysis; rice; transcriptomics
资金
- Basic Science Research Program through National Research Foundation of Korea (NRF) - Ministry of Education, Science, and Technology [2013R1A1A1A05005407]
- Next-Generation BioGreen 21 Program (Plant Molecular Breeding Center), Rural Development Administration, Republic of Korea [PJ008021]
- National Research Foundation of Korea [2013R1A1A1A05005407] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Rice blast disease caused by Magnaporthe oiyzae is one of the most serious diseases of cultivated rice (Oryza saliva L.) in most rice-growing regions of the world. In order to investigate early response genes in rice, we utilized the transcriptome analysis approach using a 300 K tilling microarray to rice leaves infected with compatible and incompatible M. oryzae strains. Prior to the microarray experiment, total RNA was validated by measuring the differential expression of rice defense-related marker genes (chitinase 2, barwin, PBZ1, and PR-10) by RT-PCR, and phytoalexins (sakuranetin and momilactone A) with HPLC. Microarray analysis revealed that 231 genes were up-regulated (>2 fold change, p < 0.05) in the incompatible interaction compared to the compatible one. Highly expressed genes were functionally characterized into metabolic processes and oxidation-reduction categories. The oxidative stress response was induced in both early and later infection stages. Biotic stress overview from MapMan analysis revealed that the phytohormone ethylene as well as signaling molecules jasmonic acid and salicylic acid is important for defense gene regulation. WRICY and Myb transcription factors were also involved in signal transduction processes. Additionally, receptor-like kinases were more likely associated with the defense response, and their expression patterns were validated by RT-PCR. Our results suggest that candidate genes, including receptor-like protein kinases, may play a key role in disease resistance against M. oryzae attack.
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