期刊
PLANT PATHOLOGY
卷 59, 期 3, 页码 443-452出版社
WILEY
DOI: 10.1111/j.1365-3059.2009.02229.x
关键词
epidemiology; hydroponics; Lycopersicon esculentum; plant-pathogen interaction; Solanum lycopersicum; water transmission
资金
- Federal Ministry of Food, Agriculture, and Consumer Protection of the Federal Republic of Germany
- Ministries of Agriculture of the States of Brandenburg and Thuringen
Pepino mosaic virus (PepMV) was shown to be efficiently transmitted between tomato plants grown in a closed recirculating hydroponic system. PepMV was detected in all plant parts after transmission via contaminated nutrient solution using ELISA, immunocapture RT-PCR, RT-PCR, electron microscopy, and by inoculation to indicator plants. Detection of PepMV in nutrient solution was only possible after concentration by ultracentrifugation followed by RT-PCR. Roots tested positive for PepMV 1-3 weeks after inoculation, and subsequently a rapid spread from the roots into the young leaves and developing fruits was found within 1 week. PepMV was only occasionally detected in the older leaves. None of the infected plants showed any symptoms on fruits, leaves or other organs. Pre-infection of roots of tomato cv. Hildares with Pythium aphanidermatum significantly delayed PepMV root infections. When mechanically inoculated with PepMV at the 2-4 leaf stage, yield loss was observed in all plants. However, only plants of cv. Castle Rock recorded significant yield losses when infected via contaminated nutrient solution. Yield losses induced by infection with PepMV and/or P. aphanidermatum ranged from 0 4 up to 40% depending on experimental conditions.
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