期刊
PLANT MOLECULAR BIOLOGY REPORTER
卷 32, 期 6, 页码 1103-1113出版社
SPRINGER
DOI: 10.1007/s11105-014-0720-6
关键词
Banana fruit; LBD transcriptional factor; EXPANSIN; Transcriptional activation; Fruit ripening
资金
- National Natural Science Foundation of China [31372111]
- China Agriculture Research System [CARS-32-09]
- Guangdong Modern Agricultural Industry Technology System [LNSG2011-12]
The LATERAL ORGAN BOUNDARIES (LOB) DOMAIN (LBD) proteins are plant-specific transcriptional factors (TFs) functioning in the growth and development of Arabidopsis and other plant species. However, the involvement of LBD TFs in regulating the ripening of economically important fruits is largely unknown. In the present study, four full-length LBD genes, designated as MaLBD1-MaLBD4, were isolated and characterized from banana fruit. Expressions of MaLBD1-MaLBD4 in fruit with three ripening characteristics revealed that MaLBD1/2/3 were ethylene-inducible, and their transcript levels obviously increased during fruit ripening, while MaLBD4 changed slightly. Moreover, the activities of MaLBD1/2/3 promoters were activated after ethylene treatment, further supporting their involvement in fruit ripening. Subcellular localization showed that MaLBD1/2/3 were nuclear proteins, and a transactivation assay in protoplasts demonstrated that MaLBD1/2/3 had transactivation activity. More importantly, a transient expression assay further indicated that MaLBD1/2/3 were transcriptional activators that regulated ripening-related MaEXP1/2 expressions by directly binding to MaEXP1/2 promoters. These results suggest that MaLBDs are involved in regulating fruit ripening, in part by transcriptional activation of the EXPANSIN expression related to cell wall modification. Taken together, our findings provide some new information about the functions of LBD TFs involved in the regulation of fruit ripening.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据