期刊
PLANT MOLECULAR BIOLOGY REPORTER
卷 29, 期 1, 页码 35-42出版社
SPRINGER
DOI: 10.1007/s11105-010-0205-1
关键词
Terpene synthase; Expression; HcTPS2; Hedychium coronarium
资金
- National Natural Science Foundation of China [30972026]
- Natural Science Foundation of Guangdong Province [07006667]
The Hedychium coronarium can emit a strong scent which is mainly composed of monoterpenes. A cDNA clone, HcTPS2 (H. coronarium terpene synthases), was cloned from H. coronarium flower. The gene has an open reading frame of 1,788 bp which encodes a protein of 596 amino acids with a calculated molecular mass of 66.7 kDa. The deduced amino acid sequence shows 35-38% identity with known monoterpene synthases in other angiosperm species. HcTPS2 was appreciably expressed in the petals, sepals, and stamens of H. coronarium, whereas no expression signal was detected in those of nonscented species. To the best of our knowledge, this is for the first time to clone the terpene synthase gene from H. coronarium, which provides the basis for biotechnological manipulation of scent composition in H. coronarium.
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