期刊
PLANT MOLECULAR BIOLOGY REPORTER
卷 29, 期 2, 页码 489-497出版社
SPRINGER
DOI: 10.1007/s11105-010-0252-7
关键词
Allene oxide cyclase; Artemisia annua L.; Jasmonate biosynthetic pathway
资金
- China 973 Program
- China 863 Program
- Ministry of Education
- Shanghai Science and Technology Committee
- Shanghai Leading Academic Discipline Project [B209]
Artemisinin is currently the best therapeutic against both drug-resistant and cerebral-malaria-causing strains of Plasmodium falciparum. Allene oxide cyclase (AOC) is the key enzyme in the biosynthetic pathway of jasmonates. In this study, a full-length cDNA of AOC gene (named as AaAOC) was cloned from Artemisia annua L. AaAOC was 1,007 bp, containing an open reading frame (750 bp) encoding 249 amino acids. Comparative and bioinformatic analyses revealed that the deduced protein of AaAOC was highly homologous to AOC from other plant species. Phylogenetic analysis indicated that AaAOC was clustered in a closely related subgroup with AOC of Camptotheca acuminata. Southern blot analysis revealed that AaAOC was a multicopy gene. Reverse transcriptase-polymerase chain reaction (RT-PCR) and QPCR analysis showed that AaAOC mRNA accumulated most abundantly in alabastrums, in which the content of artemisinin was previously proven to be the highest. RT-PCR analysis revealed that MeJA, ABA, and ethylene treatments significantly enhanced AaAOC transcript expression. And the results of HPLC showed that the contents of artemisinin were greatly increased after the treatments of ABA and MeJA.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据