期刊
PLANT MOLECULAR BIOLOGY REPORTER
卷 28, 期 1, 页码 144-151出版社
SPRINGER
DOI: 10.1007/s11105-009-0130-3
关键词
Lateral suppressor-like gene DgLsL; Shoot branching; Chrysanthemum; Transformation; IAA; GA
资金
- Program for New Century Excellent Talents in University
- Ministry of Education of the People's Republic of China [NCET-06-0489]
- Program of the Ministry of Science and Technology of the People's Republic of China [2006BAD01A1806]
- Ministry of Agriculture of People's Republic of China [2008-G3]
The lateral suppressor-like gene DgLsL was transformed by agroinfection into chrysanthemum in both the sense and antisense directions. Sense transformants branched more profusely than the wild-type nontransformant, while branching in the antisense transformants was significantly suppressed. An analysis of DgLsL transcript abundance in the shoot tips revealed that expression was enhanced in the sense transformants and suppressed in the antisense ones. The shoot tip content of indole-3-acetic acid (IAA) was reduced in the sense transformants but enhanced in the antisense ones. The sense transformants had a lower content and the antisense transformants had a higher content of gibberellic acid (GA). Cytokinin content was not affected by the variation in DgLsL expression. We conclude that DgLsL controls shoot branching through its effect on IAA and GA levels.
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