期刊
PLANT MOLECULAR BIOLOGY REPORTER
卷 27, 期 4, 页码 431-438出版社
SPRINGER
DOI: 10.1007/s11105-009-0095-2
关键词
Tomato; Growth habit; RAPD; SCAR
资金
- National Basic Research Program [2004CB117305]
- National Natural Science Foundation of China [30471108]
A mutant tomato cultivar (HT10-3-2) with indeterminate growth habit was obtained from a cultivar (T10-3-2) with determinate growth habit. The character of indeterminate growth habit in HT10-3-2 could be inherited stably. Unlike other normal growth habit cultivars, which are controlled by the SELF PRUNING gene, it was shown here that HT10-3-2 has no mutation in the sp gene. Two hundred random amplified polymorphic DNA (RAPD) primers were used to screen for polymorphism between the two genotypes from genomic DNA, and a polymorphic fragment (S168(1458)) was obtained and subsequently sequenced. However, Basic Local Alignment Search Tool searches indicated that the sequence of this RAPD fragment shares no significant homology with known sequences in GenBank. The RAPD marker (S168(1458)) was converted to a sequence characterized amplified region (SCAR) marker, SCA168(1453). The SCAR marker was tested using an F-2 population derived from the cross of T10-3-2 and HT10-3-2 and the results showed that this marker was closely linked with the unknown factor influencing the growth habit in HT10-3-2.
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