期刊
PLANT MOLECULAR BIOLOGY REPORTER
卷 27, 期 3, 页码 321-333出版社
SPRINGER
DOI: 10.1007/s11105-008-0084-x
关键词
ATHK1; Drought and salt stress; Drought and salt tolerance; Osmotic adjustment; Transgenic Lycium barbarum
资金
- National Natural Science Foundation of China [30771091]
- Chinese Ministry of Education [307024]
- Plan of Talent Scout of Lanzhou University [2004]
ATHK1 has been implicated in drought and salt tolerance in Arabidopsis thaliana. In this study, the full-length coding sequence of ATHK1 was introduced into Lycium barbarum L. by Agrobacterium transformation. Our results indicated that the transgenic plants tolerated high concentrations of NaCl or water deprivation and exhibited faster recovery following re-watering compared to wild type plants. Salt- or water-stressed transgenic plants had higher relative water content, proline and soluble protein levels, and lower chlorophyll losses and membrane ion leakage. In addition, they showed higher capacity for antioxidative reactions reflected by reduced hydrogen peroxide (H2O2), superoxide anion radical (O-2 (-)), and lipid peroxide production and increased superoxide dismutase, catalase, and peroxidase activities. The ATHK1 transcript, as shown by reverse transcription polymerase chain reaction, was more abundant under high than low osmolarity in transgenic plants. ATHK1 therefore improved tolerance of L. barbarum to drought and salt stress.
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