期刊
PLANT MOLECULAR BIOLOGY
卷 85, 期 1-2, 页码 107-121出版社
SPRINGER
DOI: 10.1007/s11103-014-0172-2
关键词
Soybean; Glycine max; Plant pathogen; Syntaxin; BOTRYTIS INDUCED KINASE 1; ENHANCED SUSCEPTIBILITY1; NONEXPRESSOR OF PR1; rhg1; Xyloglucan endotransglycosylase/hydrolase, nematode
资金
- College of Arts and Sciences (DBS)
- Mississippi Agricultural and Forestry Experimental Station (MAFES)
- competitive Special Research Initiative grant by MAFES
- Office of Research and Economic Development, Mississippi State University through a competitive Research Initiation Program grant
A Glycine max syntaxin 31 homolog (Gm-SYP38) was identified as being expressed in nematode-induced feeding structures known as syncytia undergoing an incompatible interaction with the plant parasitic nematode Heterodera glycines. The observed Gm-SYP38 expression was consistent with prior gene expression analyses that identified the alpha soluble NSF attachment protein (Gm-alpha-SNAP) resistance gene because homologs of these genes physically interact and function together in other genetic systems. Syntaxin 31 is a protein that resides on the cis face of the Golgi apparatus and binds alpha-SNAP-like proteins, but has no known role in resistance. Experiments presented here show Gm-alpha-SNAP overexpression induces Gm-SYP38 transcription. Overexpression of Gm-SYP38 rescues G. max ([Williams 82/PI 518671]), genetically rhg1 (-/-), by suppressing H. glycines parasitism. In contrast, Gm-SYP38 RNAi in the rhg1 (+/+) genotype G. max ([Peking/PI 548402]) increases susceptibility. Gm-alpha-SNAP and Gm-SYP38 overexpression induce the transcriptional activity of the cytoplasmic receptor-like kinase BOTRYTIS INDUCED KINASE 1 (Gm-BIK1-6) which is a family of defense proteins known to anchor to membranes through a 5' MGXXXS/T(R) N-myristoylation sequence. Gm-BIK1-6 had been identified previously by RNA-seq experiments as expressed in syncytia undergoing an incompatible reaction. Gm-BIK1-6 overexpression rescues the resistant phenotype. In contrast, Gm-BIK1-6 RNAi increases parasitism. The analysis demonstrates a role for syntaxin 31-like genes in resistance that until now was not known.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据