期刊
PLANT MOLECULAR BIOLOGY
卷 74, 期 6, 页码 537-547出版社
SPRINGER
DOI: 10.1007/s11103-010-9694-4
关键词
NtMAP65-1c; Microtubules-associated protein; Microtubule stabilization; Katanin
资金
- National Basic Research Program of China [2006CB100101]
- 111 Project [B06003]
- National Natural Science Foundation of China [30770186, 31070258, 30830058, 30721062]
- Chinese Universities Scientific Fund [2009JS72]
Three genes that encode MAP65-1 family proteins have been identified in the Nicotiana tabacum genome. In this study, NtMAP65-1c fusion protein was shown to bind and bundle microtubules (MTs). Further in vitro investigations demonstrated that NtMAP65-1c not only alters MT assembly and nucleation, but also exhibits high MT stabilizing activity against cold or katanin-induced destabilization. Analysis of NtMAP65-1c-GFP expressing BY-2 cells clearly demonstrated that NtMAP65-1c was able to bind to MTs during specific stages of the cell cycle. Furthermore, in vivo, NtMAP65-1c-GFP-bound cortical MTs displayed an increase in resistance against the MT-disrupting drug, propyzamide, as well as against cold temperatures. Taken together, these results strongly suggest that NtMAP65-1c stabilizes MTs and is involved in the regulation of MT organization and cellular dynamics.
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