期刊
PLANT MOLECULAR BIOLOGY
卷 74, 期 1-2, 页码 91-103出版社
SPRINGER
DOI: 10.1007/s11103-010-9656-x
关键词
Cutin; Epicuticular layer; GDSL lipase; Permeability; Rice; Wax
资金
- Crop Functional Genomic Center
- 21st Century Frontier Program [CG1111]
- Biogreen 21 Program [034-001-007-03-00]
- Rural Development Administration
- Korea Research Foundation [KRF-2007-341-C00028]
- Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea [309, 017-5]
- Forschungsgemeinschaft (DFG)
- National Research Foundation of Korea [2007-341-C00028] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Epidermal cell layers play important roles in plant defenses against various environmental stresses. Here we report the identification of a cuticle membrane mutant, wilted dwarf and lethal 1 (wdl1), from a rice T-DNA insertional population. The muant is dwarf and die at seedling stage due to increased rates of water loss. Stomatal cells and pavement cells are smaller in the mutant, suggesting that WDL1 affects epidermal cell differentiation. T-DNA was inserted into a gene that encodes a protein belonging to the SGNH subfamily, within the GDSL lipase superfamily. The WDL1-sGFP signal coincided with the RFP signal driven by AtBIP-mRFP, indicating that WDL1 is an ER protein. SEM analyses showed that their leaves have a disorganized crystal wax layer. Cross-sectioning reveals loose packing of the cuticle and irregular thickness of cell wall. Detailed analyses of the epicuticular wax showed no significant changes either in the total amount and amounts of each monomer or in the levels of lipid polymers, including cutin and other covalently bound lipids, attached to the cell wall. We propose that WDL1 is involved in cutin organization, affecting depolymerizable components.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据