期刊
PLANT MOLECULAR BIOLOGY
卷 74, 期 4-5, 页码 353-365出版社
SPRINGER
DOI: 10.1007/s11103-010-9679-3
关键词
Corn; Puroindoline; Oil content; Germ size; Transformation
资金
- Consortium for Plant Biotechnology Research
- Dow AgroSciences
- USDA-ARS [2004-35301-14538, 2007-35301-18135]
- Montana Agricultural Experiment Station
- National Science Foundation [IBN-0444031, IOS-0815104, EPS 0236913, MCB 0455318, DBI 0521587]
- USDA [2006-35100-17220, 2008-35318-18649]
- Kansas Technology Enterprise Corporation
- K-IDeA Networks of Biomedical Research Excellence (INBRE) of National Institute of Health [P20RR16475]
- Kansas State University
Plant oil content and composition improvement is a major goal of plant breeding and biotechnology. The Puroindoline a and b (PINA and PINB) proteins together control whether wheat seeds are soft or hard textured and share a similar structure to that of plant non-specific lipid-transfer proteins. Here we transformed corn (Zea mays L.) with the wheat (Triticum aestivum L.) puroindoline genes (Pina and Pinb) to assess their effects upon seed oil content and quality. Pina and Pinb coding sequences were introduced into corn under the control of a corn Ubiquitin promoter. Three Pina/Pinb expression positive transgenic events were evaluated over two growing seasons. The results showed that Pin expression increased germ size significantly without negatively impacting seed size. Germ yield increased 33.8% while total seed oil content was increased by 25.23%. Seed oil content increases were primarily the result of increased germ size. This work indicates that higher oil content corn hybrids having increased food or feed value could be produced via puroindoline expression.
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