期刊
PLANT MOLECULAR BIOLOGY
卷 69, 期 1-2, 页码 33-46出版社
SPRINGER
DOI: 10.1007/s11103-008-9403-8
关键词
Quantitative PCR; Salt tolerant; Two-dimension electrophoresis; V-H plus -ATPase; Wheat
资金
- Hebei Provincial Natural Science Fund [2007000250]
The root microsomal proteomes of salt-tolerant and salt-sensitive wheat lines under salt stress were analyzed by two-dimensional electrophoresis and mass spectrum. A wheat V-H+-ATPase E subunit protein was obtained whose expression was enhanced by salt stress. In silicon cloning identified the full-length cDNA sequences of nine subunits and partial cDNA sequences of two subunits of wheat V-H+-ATPase. The expression profiles of these V-H+-ATPase subunits in roots and leaves of both salt-tolerant and salt-sensitive wheat lines under salt and abscisic acid (ABA) stress were analyzed. The results indicate that the coordinated enhancement of the expression of V-H+-ATPase subunits under salt and ABA stress is an important factor determining improved salt tolerance in wheat. The expression of these subunits was tissue-specific. Overexpression of the E subunit by transgenic Arabidopsis thaliana was able to enhance seed germination, root growth and adult seedling growth under salt stress.
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