期刊
PLANT JOURNAL
卷 79, 期 1, 页码 173-180出版社
WILEY
DOI: 10.1111/tpj.12546
关键词
systems biology; combined isolation; RNA; small RNA; proteins; metabolites; Chlamydomonas reinhardtii; Arabidopsis thaliana; Populus sp.; Pinus sp.; technical advance
资金
- National Funds through Portuguese Science Foundation (FCT) within project EXPL/AAG [TEC/2056/2013, PEst-C/MAR/LA0017/2013]
- European Union
- COMPETE
- MEYS CR Re. [CZ.1.07/2.3.00/20.0256]
- FCT [SFRH/BPD/89765/2012]
- EU [CZ.1.07/2.3.00/20.0256]
- Severo Ochoa Programme (Gobierno del Principado de Asturias, Spain) [BP11117]
- Spanish Government (Juan de la Cierva Programme) [JCI-2011-08958]
- EU-Marie-Curie ITN MERIT [GA 2010-264474]
- Fundação para a Ciência e a Tecnologia [EXPL/AAG-TEC/2056/2013, SFRH/BPD/89765/2012] Funding Source: FCT
Here, we describe a method for the combined metabolomic, proteomic, transcriptomic and genomic analysis from one single sample as a major step for multilevel data integration strategies in systems biology. While extracting proteins and DNA, this protocol also allows the separation of metabolites into polar and lipid fractions, as well as RNA fractionation into long and small RNAs, thus allowing a broad range of transcriptional studies. The isolated biomolecules are suitable for analysis with different methods that range from electrophoresis and blotting to state-of-the-art procedures based on mass spectrometry (accurate metabolite profiling, shot-gun proteomics) or massive sequencing technologies (transcript analysis). The low amount of starting tissue, its cost-efficiency compared with the utilization of commercial kits, and its performance over a wide range of plant, microbial, and algal species such as Chlamydomonas, Arabidopsis, Populus, or Pinus, makes this method a universal alternative for multiple molecular isolation from plant tissues.
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