期刊
PLANT JOURNAL
卷 77, 期 4, 页码 568-576出版社
WILEY
DOI: 10.1111/tpj.12402
关键词
clear native electrophoresis; Hordeum vulgare; single particle electron microscopy; PSI-NDH supercomplex; cyclic electron transport
资金
- Ministry of Education, Youth and Sports of the Czech Republic [ED0007/01/01, CZ.1.07/2.3.00/20.0057]
- Grant Agency of the Czech Republic [13-28093S/P501]
- Marie Curie Career Integration Grant [322139]
Cyclic electron transport (CET) around photosystem I (PSI) plays an important role in balancing the ATP/NADPH ratio and the photoprotection of plants. The NAD(P)H dehydrogenase complex (NDH) has a key function in one of the CET pathways. Current knowledge indicates that, in order to fulfill its role in CET, the NDH complex needs to be associated with PSI; however, until now there has been no direct structural information about such a supercomplex. Here we present structural data obtained for a plant PSI-NDH supercomplex. Electron microscopy analysis revealed that in this supercomplex two copies of PSI are attached to one NDH complex. A constructed pseudo-atomic model indicates asymmetric binding of two PSI complexes to NDH and suggests that the low-abundant Lhca5 and Lhca6 subunits mediate the binding of one of the PSI complexes to NDH. On the basis of our structural data, we propose a model of electron transport in the PSI-NDH supercomplex in which the association of PSI to NDH seems to be important for efficient trapping of reduced ferredoxin by NDH.
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