期刊
PLANT JOURNAL
卷 78, 期 3, 页码 385-397出版社
WILEY
DOI: 10.1111/tpj.12483
关键词
rice; reproduction; lincRNA; phasiRNA; Argonaute
资金
- JSPS KAKENHI [21678001, 25252004]
- JSPS Research Fellowships for Young Scientists
- Charitable Trust HAYASHI Female Physical Philosopher Research Support
- Grants-in-Aid for Scientific Research [21678001, 25252004, 26870796] Funding Source: KAKEN
Small RNAs that interact with Argonaute (AGO) proteins play central roles in RNA-mediated silencing. MEIOSIS ARRESTED AT LEPTOTENE1 (MEL1), a rice AGO, has specific functions in the development of pre-meiotic germ cells and the progression of meiosis. Here, we show that MEL1, which is located mostly in the cytoplasm of germ cells, associates preferentially with 21-nucleotide phased small interfering RNAs (phasiRNAs) that bear a 5 '-terminal cytosine. Most phasiRNAs are derived from 1171 intergenic clusters distributed on all rice chromosomes. From these clusters, over 700 large intergenic, non-coding RNAs (lincRNAs) that contain the consensus sequence complementary to miR2118 are transcribed specifically in inflorescences, and cleaved within the miR2118 site. Cleaved lincRNAs are processed via DICER-LIKE4 (DCL4) protein, resulting in production of phasiRNAs. This study provides the evidence that the miR2118-dependent and the DCL4-dependent pathways are both required for biogenesis of 21-nt phasiRNAs associated with germline-specific MEL1 AGO in rice, and over 700 lincRNAs are key factors for induction of this biogenesis during reproductive-specific stages.
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