期刊
PLANT JOURNAL
卷 80, 期 6, 页码 951-964出版社
WILEY
DOI: 10.1111/tpj.12680
关键词
LRR kinase; H+ pump; peptide signalling; apoplastic pH; cell elongation
资金
- Center for Membrane Pumps in Cells and Disease-PUMPKIN, Danish National Research Foundation
- Villum Kann Rasmussen grant [VKRB06-061]
- Danish Council for Independent Research, Natural Sciences (FNU) grant [0602-00971B]
- Australian Research Council
Acidification of the cell wall space outside the plasma membrane is required for plant growth and is the result of proton extrusion by the plasma membrane-localized H+-ATPases. Here we show that the major plasma membrane proton pumps in Arabidopsis, AHA1 and AHA2, interact directly in vitro and in planta with PSY1R, a receptor kinase of the plasma membrane that serves as a receptor for the peptide growth hormone PSY1. The intracellular protein kinase domain of PSY1R phosphorylates AHA2/AHA1 at Thr-881, situated in the autoinhibitory region I of the C-terminal domain. When expressed in a yeast heterologous expression system, the introduction of a negative charge at this position caused pump activation. Application of PSY1 to plant seedlings induced rapid in planta phosphorylation at Thr-881, concomitant with an instantaneous increase in proton efflux from roots. The direct interaction between AHA2 and PSY1R observed might provide a general paradigm for regulation of plasma membrane proton transport by receptor kinases.
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