期刊
PLANT JOURNAL
卷 80, 期 5, 页码 926-935出版社
WILEY-BLACKWELL
DOI: 10.1111/tpj.12689
关键词
MALDI-MS imaging; cutin; suberin; in situ degradation; relative quantification; Solanum lycopersicum; Prunus persica; Malus domestica; GC-MS; technical advance
资金
- INRA (Institut National de Recherche Agronomique, France)
- AgreenSkills [PCOFUND-GA-2010-267-196]
The cutin polymers of different fruit cuticles (tomato, apple, nectarine) were examined using matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) after in situ release of the lipid monomers by alkaline hydrolysis. The mass spectra were acquired from each coordinate with a lateral spatial resolution of approximately 100m. Specific monomers were released at their original location in the tissue, suggesting that post-hydrolysis diffusion can be neglected. Relative quantification of the species was achieved by introducing an internal standard, and the collection of data was subjected to non-supervised and supervised statistical treatments. The molecular images obtained showed a specific distribution of ions that could unambiguously be ascribed to cutinized and suberized regions observed at the surface of fruit cuticles, thus demonstrating that the method is able to probe some structural changes that affect hydrophobic cuticle polymers. Subsequent chemical assignment of the differentiating ions was performed, and all of these ions could be matched to cutin and suberin molecular markers. Therefore, this MALDI-MSI procedure provides a powerful tool for probing the surface heterogeneity of plant lipid polymers. This method should facilitate rapid investigation of the relationships between cuticle phenotypes and the structure of cutin within a large population of mutants.
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