期刊
PLANT JOURNAL
卷 76, 期 4, 页码 718-727出版社
WILEY
DOI: 10.1111/tpj.12319
关键词
next-generation sequencing; genome assembly; genetic mapping; barley; Hordeum vulgare; population sequencing; technical advance
资金
- Office of Science of the US Department of Energy [DE-AC02-05CH11231]
- Triticeae Coordinated Agricultural Project, US Department of Agriculture/National Institute for Food and Agriculture [2011-68002-30029]
- Scottish Government Rural and Environment Science and Analytical Services Division Research Programme
- Bundesministerium fur Bildung und Forschung [TRI-TEX 0315954]
- Biotechnology and Biological Sciences Research Council [BBS/E/T/000PR6193, BB/I00663X/1] Funding Source: researchfish
- BBSRC [BB/I00663X/1, BBS/E/T/000PR6193] Funding Source: UKRI
Next-generation whole-genome shotgun assemblies of complex genomes are highly useful, but fail to link nearby sequence contigs with each other or provide a linear order of contigs along individual chromosomes. Here, we introduce a strategy based on sequencing progeny of a segregating population that allows de novo production of a genetically anchored linear assembly of the gene space of an organism. We demonstrate the power of the approach by reconstructing the chromosomal organization of the gene space of barley, a large, complex and highly repetitive 5.1Gb genome. We evaluate the robustness of the new assembly by comparison to a recently released physical and genetic framework of the barley genome, and to various genetically ordered sequence-based genotypic datasets. The method is independent of the need for any prior sequence resources, and will enable rapid and cost-efficient establishment of powerful genomic information for many species.
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