期刊
PLANT JOURNAL
卷 77, 期 1, 页码 160-172出版社
WILEY-BLACKWELL
DOI: 10.1111/tpj.12365
关键词
Arabidopsis thaliana; mass spectrometry; lipidomics; seed oil; triacylglycerol profiling; technical advance
资金
- United States Department of Energy (DOE), Office of Science, Office of Basic Energy Sciences (BES), Materials Sciences and Engineering Division [DE-SC0001295]
- National Science Foundation [MCB-0922879, MCB-0920663, DBI-0521587]
- Kansas Experimental Program to Stimulate Competitive Research Award [EPS-0236913]
- Kansas Agricultural Experiment Station [14-035-J]
- Direct For Biological Sciences
- Div Of Molecular and Cellular Bioscience [0920663] Funding Source: National Science Foundation
- Direct For Biological Sciences
- Div Of Molecular and Cellular Bioscience [0920681] Funding Source: National Science Foundation
Plant triacylglycerols (TAGs), or vegetable oils, provide approximately 25% of dietary calories to humans and are becoming an increasingly important source of renewable bioenergy and industrial feedstocks. TAGs are assembled by multiple enzymes in the endoplasmic reticulum from building blocks that include an invariable glycerol backbone and variable fatty acyl chains. It remains a challenge to elucidate the mechanism of synthesis of hundreds of different TAG species in planta. One reason is the lack of an efficient analytical approach quantifying individual molecular species. Here we report a rapid and quantitative TAG profiling approach for Arabidopsis seeds based on electrospray ionization tandem mass spectrometry with direct infusion and multiple neutral loss scans. The levels of 93 TAG molecular species, identified by their acyl components, were determined in Arabidopsis seeds. Quantitative TAG pattern analyses revealed that the TAG assembly machinery preferentially produces TAGs with one elongated fatty acid. The importance of the selectivity in oil synthesis was consistent with an observation that an Arabidopsis mutant overexpressing a patatin-like phospholipase had enhanced seed oil content with elongated fatty acids. This quantitative TAG profiling approach should facilitate investigations aimed at understanding the biochemical mechanisms of TAG metabolism in plants.
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