期刊
PLANT JOURNAL
卷 76, 期 6, 页码 914-929出版社
WILEY-BLACKWELL
DOI: 10.1111/tpj.12342
关键词
leaf; development; cell cycle; physiology; imaging; Arabidopsis thaliana
资金
- EU-Transfer of Knowledge grant Generating an Integrated Plant Science [42236]
- UK Biotechnology and Biological Sciences Research Council award [BB/J0004065]
- BBSRC [BB/J004030/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/J004030/1] Funding Source: researchfish
The causal relationship between cell division and growth in plants is complex. Although altered expression of cell-cycle genes frequently leads to altered organ growth, there are many examples where manipulation of the division machinery leads to a limited outcome at the level of organ form, despite changes in constituent cell size. One possibility, which has been under-explored, is that altered division patterns resulting from manipulation of cell-cycle gene expression alter the physiology of the organ, and that this has an effect on growth. We performed a series of experiments on retinoblastoma-related protein (RBR), a well characterized regulator of the cell cycle, to investigate the outcome of altered cell division on leaf physiology. Our approach involved combination of high-resolution microCT imaging and physiological analysis with a transient gene induction system, providing a powerful approach for the study of developmental physiology. Our investigation identifies a new role for RBR in mesophyll differentiation that affects tissue porosity and the distribution of air space within the leaf. The data demonstrate the importance of RBR in early leaf development and the extent to which physiology adapts to modified cellular architecture resulting from altered cell-cycle gene expression.
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