期刊
PLANT JOURNAL
卷 76, 期 1, 页码 36-46出版社
WILEY
DOI: 10.1111/tpj.12268
关键词
Oryza sativa L; flowering time; photoperiod sensitivity; natural variation; casein kinase I
资金
- Ministry of Agriculture, Forestry and Fisheries of Japan (Integrated Research Project for Plant, Insect, and Animal using Genome Technology) [IP1001, GPN0001]
- Ministry of Education, Culture, Sports, Science and Technology of Japan [20780008]
- Grants-in-Aid for Scientific Research [20780008] Funding Source: KAKEN
The alteration of photoperiod sensitivity has let breeders diversify flowering time in Oryza sativa (rice) and develop cultivars adjusted to a range of growing season periods. Map-based cloning revealed that the rice flowering-time quantitative trait locus (QTL) Heading date 16 (Hd16) encodes a casein kinase-I protein. One non-synonymous substitution in Hd16 resulted in decreased photoperiod sensitivity in rice, and this substitution occurred naturally in an old rice cultivar. By using near-isogenic lines with functional or deficient alleles of several rice flowering-time genes, we observed significant digenetic interactions between Hd16 and four other flowering-time genes (Ghd7, Hd1, DTH8 and Hd2). In a near-isogenic line with the weak-photoperiod-sensitivity allele of Hd16, transcription levels of Ehd1, Hd3a, and RFT1 increased under long-day conditions, and transcription levels of Hd3a and RFT1 decreased under short-day conditions. Expression analysis under continuous light and dark conditions showed that Hd16 was not likely to be associated with circadian clock regulation. Biochemical characterization indicated that the functional Hd16 recombinant protein specifically phosphorylated Ghd7. These results demonstrate that Hd16 acts as an inhibitor in the rice flowering pathway by enhancing the photoperiod response as a result of the phosphorylation of Ghd7.
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