期刊
PLANT JOURNAL
卷 72, 期 4, 页码 652-661出版社
WILEY-BLACKWELL
DOI: 10.1111/j.1365-313X.2012.05111.x
关键词
chloroplast biogenesis; translation; photosystem I; Chlamydomonas reinhardtii; OPR protein
资金
- Swiss National Foundation [31003A_133089/1]
- Swiss National Science Foundation (SNF) [31003A_133089] Funding Source: Swiss National Science Foundation (SNF)
Genetic analysis of mutants deficient in the biosynthesis of the photosystem I complex has revealed several nucleus-encoded factors that act at different post-transcriptional steps of chloroplast gene expression. Here we have identified and characterized the gene affected in the tab 1-F15 mutant, which is specifically deficient in the translation of the photosystem I reaction center protein PsaB as the result of a single nucleotide deletion. This gene encodes Tab 1, a 1287 amino acid protein that contains 10 tandem 3840 amino acid degenerate repeats of the PPPEW/OPR (octatricopeptide repeat) family, first described for the chloroplast translation factor Tbc2. These repeats are involved in the binding of Tab 1 to the 5'-untranslated region of the psaB mRNA based on gel mobility shift assays. Tab 1 is part of a large family of proteins in Chlamydomonas that are also found in several bacteria and protozoans, but are rare in land plants.
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