期刊
PLANT JOURNAL
卷 67, 期 5, 页码 929-940出版社
WILEY
DOI: 10.1111/j.1365-313X.2011.04645.x
关键词
legumes; root; symbiosis; SYMRK; signalling; calcium
资金
- Agriculture and Agri-Food Canada Crop Genomics Initiative
- National Science and Engineering Research Council of Canada (NSERC) [3277A01]
- Biotechnology and Biological Sciences Research Council
- NSERC PGS-D
- Ontario Genomic Institute (OGI)
- European Union [MRTN-CT-2006-035546]
- German Research Council (DFG) [SPP1212]
SYMRK is a leucine-rich-repeat (LRR)-receptor kinase that mediates intracellular symbioses of legumes with rhizobia and arbuscular mycorrhizal fungi. It participates in signalling events that lead to epidermal calcium spiking, an early cellular response that is typically considered as central for intracellular accommodation and nodule organogenesis. Here, we describe the Lotus japonicus symRK-14 mutation that alters a conserved GDPC amino-acid sequence in the SYMRK extracellular domain. Normal infection of the epidermis by fungal or bacterial symbionts was aborted in symRK-14. Likewise, epidermal responses of symRK-14 to bacterial signalling, including calcium spiking, NIN gene expression and infection thread formation, were significantly reduced. In contrast, no major negative effects on the formation of nodule primordia and cortical infection were detected. Cumulatively, our data show that the symRK-14 mutation uncouples the epidermal and cortical symbiotic program, while indicating that the SYMRK extracellular domain participates in transduction of non-equivalent signalling events. The GDPC sequence was found to be highly conserved in LRR-receptor kinases in legumes and non-legumes, including the evolutionarily distant bryophytes. Conservation of the GDPC sequence in nearly one-fourth of LRR-receptor-like kinases in the genome of Arabidopsis thaliana suggests, however, that this sequence might also play an important non-symbiotic function in this plant.
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