期刊
PLANT JOURNAL
卷 69, 期 5, 页码 894-905出版社
WILEY
DOI: 10.1111/j.1365-313X.2011.04841.x
关键词
Arabidopsis; aquaporins; constitutive cycling; salt stress; fluorescence recovery after photobleaching
资金
- UK Biotechnology and Biological Sciences Research Council [BB/F01407/1]
- French Ministry of Research
- Biotechnology and Biological Sciences Research Council [BB/F014074/1] Funding Source: researchfish
- BBSRC [BB/F014074/1] Funding Source: UKRI
The constitutive cycling of plant plasma membrane (PM) proteins is an essential component of their function and regulation under resting or stress conditions. Transgenic Arabidopsis plants that express GFP fusions with AtPIP1;2 and AtPIP2;1, two prototypic PM aquaporins, were used to develop a fluorescence recovery after photobleaching (FRAP) approach. This technique was used to discriminate between PM and endosomal pools of the aquaporin constructs, and to estimate their cycling between intracellular compartments and the cell surface. The membrane trafficking inhibitors tyrphostin A23, naphthalene-1-acetic acid and brefeldin A blocked the latter process. By contrast, a salt treatment (100 mm NaCl for 30 min) markedly enhanced the cycling of the aquaporin constructs and modified their pharmacological inhibition profile. Two distinct models for PM aquaporin cycling in resting or salt-stressed root cells are discussed.
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