期刊
PLANT JOURNAL
卷 66, 期 5, 页码 725-734出版社
WILEY
DOI: 10.1111/j.1365-313X.2011.04533.x
关键词
Oryzasativa L; expansin; root hair elongation; EXPA17; root hair-specific gene; disulfide bond
资金
- National Basic Research and Development Program of China [2005CB120900]
- Special Program of Rice Functional Genomics of China [2006AA10A102]
- Science and Technology Department of Zhejiang Province, China
- National Research Foundation of Korea [2011-0017242] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
P>Root hair growth requires intensive cell-wall modification. This study demonstrates that root hair-specific expansin As, a sub-clade of the cell wall-loosening expansin proteins, are required for root hair elongation in rice (Oryza sativa L.). We identified a gene encoding EXPA17 (OsEXPA17) from a rice mutant with short root hairs. Promoter::reporter transgenic lines exhibited exclusive OsEXPA17 expression in root hair cells. The OsEXPA17 mutant protein (OsexpA17) contained a point mutation, causing a change in the amino acid sequence (Gly104 -> Arg). This amino acid alteration is predicted to disrupt a highly conserved disulfide bond in the mutant. Suppression of OsEXPA17 by RNA interference further confirmed requirement for the gene in root hair elongation. Complementation of the OsEXPA17 mutant with other root hair EXPAs (OsEXPA30 and Arabidopsis EXPA7) can restore root hair elongation, indicating functional conservation of these root hair EXPAs in monocots and dicots. These results demonstrate that members of the root hair EXPA sub-clade play a crucial role in root hair cell elongation in Graminaceae.
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