期刊
PLANT JOURNAL
卷 67, 期 6, 页码 1055-1066出版社
WILEY
DOI: 10.1111/j.1365-313X.2011.04657.x
关键词
nucleo-chloroplast interactions; ATP synthase; translation regulation; ribonucleoprotein complexes; Chlamydomonas reinhardtii
资金
- Centre National de la Recherche Scientifique (CNRS)
- Universite Pierre et Marie Curie, Paris 06 (UPMC)
- Unite Mixte de Recherche (UMR) [7141]
- Agence Nationale pour la Recherche (ANR) [BLAN-NT09_451610]
- European Community [FP7-KBBE-2009-3-02]
After endosymbiosis, organelles lost most of their initial genome. Moreover, expression of the few remaining genes became tightly controlled by the nucleus through trans-acting protein factors that are required for post-transcriptional expression (maturation/stability or translation) of a single (or a few) specific organelle target mRNA(s). Here, we characterize the nucleus-encoded TDA1 factor, which is specifically required for translation of the chloroplast atpA transcript that encodes subunit a of ATP synthase in Chlamydomonas reinhardtii. The sequence of TDA1 contains eight copies of a degenerate 38-residue motif, that we named octotrico peptide repeat (OPR), which has been previously described in a few other trans-acting factors targeted to the C. reinhardtii chloroplast. Interestingly, a proportion of the untranslated atpA transcripts are sequestered into high-density, non-polysomic, ribonucleoprotein complexes. Our results suggest that TDA1 has a dual function: (i) trapping a subset of untranslated atpA transcripts into non-polysomic complexes, and (ii) translational activation of these transcripts. We discuss these results in light of our previous observation that only a proportion of atpA transcripts are translated at any given time in the chloroplast of C. reinhardtii.
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