期刊
PLANT JOURNAL
卷 58, 期 1, 页码 165-174出版社
WILEY
DOI: 10.1111/j.1365-313X.2008.03767.x
关键词
RNA silencing; artificial miRNA; oligonucleotide cloning; Chlamydomonas; functional genomics
资金
- Gatsby Charitable Foundation to the Sainsbury Laboratory and to DCB in Cambridge
- Biotechnology and Biological Sciences Research Council grant BBSRC [BB/E004091/1]
- European Union Sixth Framework Program Integrated Project SIROCCO [LSHG-CT-2006-037900]
- Marie Curie Early Stage Training Fellowship [MEST-CT-2005-019727]
- Royal Society Research Professor
- Biotechnology and Biological Sciences Research Council [BB/E004091/1, BBS/E/J/00000166] Funding Source: researchfish
- BBSRC [BB/E004091/1] Funding Source: UKRI
MicroRNAs (miRNAs) are small RNAs, 21 to 22 nucleotides long, with important regulatory roles. They are processed from longer RNA molecules with imperfectly matched foldback regions and they function in modulating the stability and translation of mRNA. Recently, we and others have demonstrated that the unicellular alga Chlamydomonas reinhardtii, like diverse multicellular organisms, contains miRNAs. These RNAs resemble the miRNAs of land plants in that they direct site-specific cleavage of target mRNA with miRNA-complementary motifs and, presumably, act as regulatory molecules in growth and development. Utilizing these findings we have developed a novel artificial miRNA system based on ligation of DNA oligonucleotides that can be used for specific high-throughput gene silencing in green algae.
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