期刊
PLANT JOURNAL
卷 57, 期 5, 页码 819-831出版社
WILEY
DOI: 10.1111/j.1365-313X.2008.03725.x
关键词
preprophase band of microtubules; endocytosis; electron tomography; clathrin-coated pits; clathrin-coated vesicles
资金
- MEXT to IK [11740454, 1474 0454]
- JSPS [12640651, 17207006]
- MEXT [17049019]
- NIH [GM61306]
- Grants-in-Aid for Scientific Research [12640651, 17049019, 17207006, 11740454] Funding Source: KAKEN
The preprophase band (PPB) marks the site on the plant cell cortex where the cell plate will fuse during the final stage of cytokinesis. Recent studies have shown that several cytoskeletal proteins are depleted at the PPB site, but the processes that bring about these changes are still unknown. We have investigated the membrane systems associated with the PPB regions of epidermal cells of onion cotyledons by means of serial thin sections and electron tomograms. In contrast with specimens preserved by chemical fixatives, our high-pressure frozen cells demonstrated the presence of large numbers of clathrin-coated pits and vesicles in the PPB regions. The vesicles were of two types: clathrin-coated and structurally related, non-coated vesicles. Quantitative analysis of the data revealed that the number of clathrin-coated pits and vesicles is higher in the PPB regions than outside of these regions. Immunofluorescent microscopy using anti-plant clathrin-antibody confirmed this result. In contrast, no differences in secretory activities were observed. We postulate that the removal of membrane proteins by endocytosis plays a role in the formation of PPB 'memory' structures.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据