期刊
PLANT JOURNAL
卷 56, 期 2, 页码 316-326出版社
WILEY-BLACKWELL
DOI: 10.1111/j.1365-313X.2008.03597.x
关键词
transcription factor; flavonoids; caffeoyl quinic acids; secondary metabolism; metabolic engineering; polyphenols
资金
- National Science Foundation of China [30500038]
- Chinese Scholarship Council
- Biological and Biotechnological Science Research Council Agri-Food Committee [218/D11645]
- EU FP6 FLORA [FOOD-CT-01730]
- Biological and Biotechnological Science Research Council
Plant polyphenolics exhibit a broad spectrum of health-promoting effects when consumed as part of the diet, and there is considerable interest in enhancing the levels of these bioactive molecules in plants used as foods. AtMYB12 was originally identified as a flavonol-specific transcriptional activator in Arabidopsis thaliana, and this has been confirmed by ectopic expression in tobacco. AtMYB12 is able to induce the expression of additional target genes in tobacco, leading to the accumulation of very high levels of flavonols. When expressed in a tissue-specific manner in tomato, AtMYB12 activates the caffeoyl quinic acid biosynthetic pathway, in addition to the flavonol biosynthetic pathway, an activity which probably mirrors that of the orthologous MYB12-like protein in tomato. As a result of its broad specificity for transcriptional activation in tomato, AtMYB12 can be used to produce fruit with extremely high levels of multiple polyphenolic anti-oxidants. Our data indicate that transcription factors may have different specificities for target genes in different plants, which is of significance when designing strategies to improve metabolite accumulation and the anti-oxidant capacity of foods.
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