期刊
PLANT GROWTH REGULATION
卷 68, 期 3, 页码 511-515出版社
SPRINGER
DOI: 10.1007/s10725-012-9726-y
关键词
Peanut; Wild species; In vitro technique; Hormone treatment; Seed development; Intersectional hybrid
资金
- China Agricultural Research System [CARS-14]
- Qingdao Science & Technology Support Program [10-3-3-20-nsh, 09-1-3-67-jch]
- Shandong Natural Science Foundation [Y2008D11]
- Shandong Key Project of Science Technology [2009GG10009008]
- Jilin Natural Science Foundation [20101580]
To broaden the narrow gene base of the cultivated peanut and utilize sources of resistance to abiotic/biotic stresses, quality-related traits and high yielding factors, much attention has been paid to wild relatives of the oilseed crop since 1980s. Species outside section Arachis are cross-incompatible with A. hypogaea L.; even some of the species from section Arachis, which are supposed to be compatible, may also encounter obstacles when crossed with the peanut cultigen. The objective of the present communication is to study the effects of simple hormone treatment on production of true incompatible hybrids in peanut to replace the tedious and lengthy in vitro embryo rescue procedures currently in use. In the incompatible cross, A. hypogaea cv Qunyu 101 x A. paraguariensis, post-pollination application of a hormone aqueous solution (IAA 4 mg/L + GA 2 mg/L) at flower bases resulted in 42 seeds, of which 26 were identified as true hybrids by allele-specific PCR for FAD2A genotyping. As a desirable alternative to in vitro embryo/ovules/peg culture, the present method may facilitate the utilization of wild species in peanut breeding. The method described here for peanut may be of reference to other crop plants where embryo abortion is also a problem.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据