期刊
PLANT FOODS FOR HUMAN NUTRITION
卷 69, 期 3, 页码 270-275出版社
SPRINGER
DOI: 10.1007/s11130-014-0426-9
关键词
Sweet potato protein; In vitro digestibility; Secondary structure; Differential scanning calorimeter; Circular dichroism
资金
- earmarked fund for China Agriculture Research System [CARS-11-B-19]
- International Science & Technology Cooperation Program of China [2010DFA32690]
- Fundamental Research Funds for the Central Public Scientific Research [2012ZL036]
This study assessed the effects of thermal (40, 60, 80, 100 and 127 A degrees C) and high hydrostatic pressure (HHP, 200, 400 and 600 MPa) treatments on the in vitro digestibility and structural properties of sweet potato protein (SPP). The results showed that the in vitro digestibility of SPP increased significantly with increasing heating temperature and heating time (0-60 min), while HHP treatment had little or no effect. Native SPP denaturation temperature (T (d) ) and enthalpy change (Delta H) were 89.0 A degrees C and 9.6 J/g, respectively. Thermal and HHP treated SPP had T (d) of 84.6-88.9 A degrees C and 86.4-87.6 A degrees C, respectively. Delta H of thermal treated SPP was 3.6-6.4 J/g, while that of HHP treated SPP was 5.9-7.8 J/g. The differential scanning calorimetry (DSC) results demonstrated that HHP and thermal treatments both significantly reduced SPP thermodynamic stability. Circular dichroism analyses revealed that native SPP contains alpha-helixes, beta-sheets and random coils (4.3, 48.0 and 47.7 %, respectively). After thermal treatment at 127 A degrees C for 20 min, the content of alpha-helixes and turns increased significantly (13.2 and 27.6 %, respectively), whereas the content of beta-sheets decreased significantly (12.3 %). In contrast, HHP treatment increased the content of beta-sheets, but decreased the content of random coils. This study suggested that the SPP structure changes might be the main reason affecting the in vitro digestibility of SPP, and thermal treatment was more effective at changing SPP secondary structures and improving in vitro SPP digestibility than HHP treatment.
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