期刊
PLANT CELL TISSUE AND ORGAN CULTURE
卷 118, 期 3, 页码 431-444出版社
SPRINGER
DOI: 10.1007/s11240-014-0495-3
关键词
Abiotic stresses; CaAQP; Capsicum annuum L.; Tonoplast intrinsic protein; Transgenic tobacco
资金
- National Natural Science Foundation of China [31201615, 31272163]
- National Science and Technology in Rural Areas [2011BAD12B03]
- Shaanxi Provincial Science and Technology Coordinating Innovative Engineering Project [2012KTCL02-09]
Ubiquitous cell membrane proteins called aquaporins are members of major intrinsic proteins (MIPs), which control the specific transport of water molecules across cell membranes. A pepper aquaporin gene (CaAQP), which exhibits the structural features of tonoplast intrinsic proteins of the MIP subfamily, was isolated from the leaves of chilling-treated seedlings of pepper (Capsicum annuum L.) cv. P70. Assays indicated high levels of expression in young seeds, green fruits and flower buds and low levels of expression in the stems, leaves and roots of pepper. The expression patterns were strongly and rapidly induced by HgCl2, low temperature, abscisic acid, fluridone and osmotic stresses. The responsiveness of pepper seedlings pretreated with abscisic acid at low temperatures demonstrated up-regulation of CaAQP by chilling, which is potentially involved in ABA signalling. Our results indicated that overexpression of CaAQP decreased chilling stress in transgenic plants, likely by increasing the stomatal aperture under stress, increasing the rate of membrane damage during the recovery stage, thereby affecting the intercellular CO2 concentration with lower stomatal conductance and transpiration rates. VIGS of CaAQP in pepper plants caused significant growth retardation. These results suggested that CaAQP played a crucial role in the plant response to abiotic stresses.
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