期刊
PLANT CELL TISSUE AND ORGAN CULTURE
卷 119, 期 1, 页码 1-13出版社
SPRINGER
DOI: 10.1007/s11240-014-0509-1
关键词
Citrus; Embryogenesis; Ectopic expression; LEAFY COTYLEDON (LEC); LEAFY COTYLEDON 1-LIKE (L1L)
资金
- National Natural Science Fundation of China [31221062, 31101521]
- CARS (China Agriculture Research System)
A member of the LEAFY COTYLEDON gene family encoding a HAP3 (heme activated protein 3) sub-unit of the CCAAT box-binding factor was isolated and termed as Citrus sinensis LEAFY COTYLEDON 1-LIKE (CsL1L). The deduced amino acid sequence shared a high similarity with LEAFY COTYLEDON 1-LIKE (L1L) in Arabidopsis thaliana, Phaseolus coccineus, Theobroma cacao, and Helianthus annuus. Quantitative RT-PCR results indicated that CsLIL was highly expressed in embryogenic callus, somatic embryos and immature seeds, but was rarely detected in non-embryogenic callus, vegetative and floral tissues. Ectopic expression of CsL1L in vegetative tissues could induce embryo-like structures, suggesting that CsL1L has the capability to transit cells from vegetative to embryogenic phase. Comparison of CsL1L expression in the newly formed and long-term subcultured embryogenic calli of W. Murcott tangor (C. sinensis x C. reticulata) and Hongkong kumquat Fortunella hindsii Swingle) revealed that the potency of embryogenesis was related to the level of CsL1L expression. Sub-cellular localization analysis indicated that CsL1L was a nuclear protein in plant. A microsatellite in CsL1L was verified with polymorphism among the citrus species.
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