期刊
PLANT CELL TISSUE AND ORGAN CULTURE
卷 111, 期 3, 页码 291-301出版社
SPRINGER
DOI: 10.1007/s11240-012-0194-x
关键词
Bacopa; Bacoside A; 'Brahmi'; Genetic stability; ISSR; Medicinal plant; Medium-term conservation; Mineral oil overlay; RAPD; Slow growth
资金
- Department of Biotechnology, Ministry of Science and Technology
Bacopa monnieri (L.) Wettst., a traditional Indian medicinal plant with high commercial potential, is used as a potent nervine tonic. A slow growth protocol was developed for medium-term conservation using mineral oil (MO) overlay. Nodal segments of B. monnieri (two genotypes; IC249250, IC468878) were conserved using MO for 24 months. Single node explants were implanted on MS medium supplemented with 0.2 mg l(-1) BA and were covered with MO. Subculture duration could be significantly enhanced from 6 to 24 months, on the above medium. Normal plants regenerated from conserved cultures were successfully established in soil. On the basis of 20 random amplified polymorphic DNA and 5 inter-simple sequence repeat primers analyses and bacoside A content using HPLC, no significant reproducible variation was observed between the controls and in vitro-conserved plants. The results demonstrate the feasibility of using MO for medium-term conservation of B. monnieri germplasm without any adverse genetical and biochemical effects.
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