期刊
PLANT CELL TISSUE AND ORGAN CULTURE
卷 109, 期 1, 页码 101-109出版社
SPRINGER
DOI: 10.1007/s11240-011-0078-5
关键词
Carrot; Daucus carota; Plating efficiency; Protoplast viability; Somatic embryogenesis
资金
- Polish Ministry of Science and Higher Education [N310 047 32/2394]
An easy and effective regeneration system from leaf- and hypocotyl-derived protoplasts was established for carrot. The protoplast isolation efficiency after preplasmolytic treatment and digestion of source material in enzyme mixture consisted of 1% cellulase Onozuka R-10 and 0.1% pectolyase Y-23 reached on average 3 x 10(6) and 10(6) protoplasts per g of leaf and hypocotyl tissue, respectively. A modified thin alginate layer technique was applied for the protoplast culture. Direct somatic embryogenesis on a simplified Kao and Michayluk medium in the presence of 2,4-D and zeatin occurred during cultivation of both leaf- and hypocotyl-derived protoplasts for all accessions used. Morphologically normal plants were produced at very high efficiency within two months after initiation of the protoplast culture. Ninety three percent of in vitro derived plants were diploids. Pollen viability and seed set after self-pollination were similar to those of plants obtained from seeds.
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