期刊
PLANT CELL TISSUE AND ORGAN CULTURE
卷 106, 期 2, 页码 235-242出版社
SPRINGER
DOI: 10.1007/s11240-010-9912-4
关键词
MdMYB6; Anthocyanins; BHLH; Sucrose; MYB
资金
- Shanghai National Natural Science Foundation [30670179, 30800602, 08ZR1417200]
- 863 Program [2006AA10Z117, 2006AA06Z358, 2008AA10Z401]
- Shanghai Project for ISTC [08540706500]
- Shanghai Municipal Committee of Agriculture [2008-7-5]
- Shanghai Rising-Star Program [08QH14021]
The R2R3-Myb proteins comprise a large family of plant transcription factors that regulate the expression of multiple drought-responsive and cold-responsive genes by binding special cis-elements at the promoter. There have been previous studies in transgenic plant over-expression of the myb protein. In this article, we analyzed the function of MdMYB6, an R2R3-type Myb transcription factor from apples (Malus x domestica), in transgenic Arabidopsis plants. In contrast to wild type plants, these transgenic lines accumulated less anthocyanin. Equimolar concentrations of sorbitol did not alter the anthocyanin accumulation. Real-time polymerase chain reaction (PCR) indicated that MdMYB6 over-expression suppressed enzymes involved in anthocyanin biosynthesis and some Arabidopsis basic helix-loop-helix (bHLH) genes. The MdMYB6 transcription faction may be an important repressor of anthocyanin biosynthesis in plants.
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