期刊
PLANT CELL TISSUE AND ORGAN CULTURE
卷 105, 期 2, 页码 219-231出版社
SPRINGER
DOI: 10.1007/s11240-010-9855-9
关键词
Ipomoea batatas; Agrobacterium tumefaciens; Genotype-independent transformation; Herbicide tolerance; Cytochrome P450
资金
- Japan Society for the Promotion of Science (JSPS) [21248001]
- Grants-in-Aid for Scientific Research [21248001] Funding Source: KAKEN
Sweet potato [Ipomoea batatas (L.) Lam] is considered to be recalcitrant to transformation and regeneration because of its genotype-dependent in vitro responses. The lack of a genotype-independent transformation and regeneration system limits biotechnological applications in this plant species. To establish a transformation system for a diverse group of sweet potato genotypes, we examined sweet potato regeneration after transformation in five cultivars. An Agrobacterium tumefaciens transformation system was used for the introduction of mammalian cytochrome P450 genes, which are capable of conferring herbicide tolerance. Among the different factors studied, including explant type, plasmid vectors, and auxin type in the initiation media, auxin type had the greatest effect on the regeneration response. Of the auxins tested, only 4-fluorophenoxyacetic acid (4FA) induced regeneration from all cultivars. In terms of the quality of calli, 4FA promoted the induction of type I calli, which were capable of somatic embryo formation, whereas type II calli fail to produce somatic embryos. The frequency of somatic embryo formation was also affected by the composition of the embryo-induction media. Transgenic plants were regenerated from all cultivars. The stable integration and expression of transgenes was confirmed by several approaches. This Agrobacterium-mediated transformation system should be applicable to a wide range of sweet potato cultivars.
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