期刊
PLANT CELL TISSUE AND ORGAN CULTURE
卷 97, 期 3, 页码 337-343出版社
SPRINGER
DOI: 10.1007/s11240-009-9522-1
关键词
Ginkgo biloba; Cell culture; Cryopreservation; Desiccation; Soluble sugars; Flow cytometry
资金
- Korean government (MOST) [R01-2007-000-10543-0]
- National Research Foundation of Korea [R01-2007-000-10543-0] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Two-years-old Ginkgo biloba cell culture initiated from cotyledonary explants was cryopreserved by a simple desiccation method. Preliminary incubation of callus clumps on MS preculture medium supplemented with 100 g l(-1) sucrose and 2 mg l(-1) ABA for 7 and 14 days resulted in accumulation of endogenous soluble sugars and was essential for cell culture post-cryopreservation survival. The optimal time for the preculture on sucrose-and-ABA containing medium was found to be 14 days. The sufficient desiccation duration was determined as 150 min. FCM profiles of calli maintained for 2 years remained stable and were not affected by cryopreservation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据